Endotoxin Testing - Microchem
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Detection of Contaminants

Endotoxin Testing

Endotoxins are potentially toxic natural compounds of the cell wall of gram negative bacteria. This material is pryrogenic meaning that it can cause high fevers in humans. The test for bacterial endotoxin is used to detect or quantify endotoxins using Limulus Amoebocyte Lysate (LAL) which is an extract of blood cells from the horseshoe crab (Limulus polyphemus).

Gel Clot Method LAL Testing

The gel clot method is based upon the reaction between bacterial endotoxin and a specific lysate. This particular lysate is derived from the circulating amebocytes associated with the blood-clotting mechanism of the horseshoe crab Limulus polyphemus. (LAL)

If a gel is formed via a clotting reaction, endotoxin is present and the sample fails the test.

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