19 Mar Meat Speciation Identification and Quantification Analyses
Microchem’s Cape Town molecular diagnostics department is in the process of offering a range of meat speciation and quantification analyses for the following animal species:
- Indian Buffalo
We are able to detect mitochorondrial DNA of specific animal origin that may be present in a food sample. The method is based on the PCR (Polymerase Chain Reaction) amplification of a unique species specific tag present in mitochondrial genome of the species under investigation. This allows for the detection of all strains of interest, whilst excluding detection of other closely related species.
Furthermore, since there are multiple copies of each mitrochondrial genome within each cell, the detection sensitivity for the analyses is up to 100 times greater than that of a test which targets a single copy locus within the nuclear DNA genome.
PCR amplification is detected by means of a double dye probe which is degraded during the PCR, releasing fluorescence. The fluorescence trace is used to both detect and quantify the number of copies of mitochondrial DNA present in the sample.
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